Fig 1. Effects of low cytosolic Mg2+ on Salmonella antibiotic tolerance.
(A) Quantification of surviving wild-type (14028s), Δ12TA (MP1422), and relA::Tn10 spoT (MP342) Salmonella following 2.5 h exposure to cefotaxime (200 μg/mL) or ciprofloxacin (2 μg/mL) following 2 or 5 h of growth in low Mg2+ medium. Error bars represent standard deviations, derived from at least 8 biological samples and 3 independent experiments. Note log scale of y axis. (B) Representative dilution series of wild-type (14028s), Δ12TA (MP1422), and relA::Tn10 spoT (MP342) Salmonella following 2.5 h exposure to cefotaxime or ciprofloxacin following 2 or 5 h of growth in low Mg2+ medium. Each spot represents a 5 μL aliquot.