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. 2020 May 7;20:197. doi: 10.1186/s12870-020-02409-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Differentially expressed genes in axillary bud under under N concentrations. a The number of up and down-regulated DEGs (fold change> 2 and padj< 0.05 by DESeq2) detected between each N stress concentrations (0.5, 1.0, 5.0, 10.0, 15.0 mM) and the optimal concentration (2.0 mM) in axillary bud. 0.5 and 1.0 mM N are low nitrogen (LN) concentrations, while 5.0, 10.0, and 15.0 mM are high nitrogen (HN) concentrations. The number of up-, down- regulated and total DEGs were shown. b Clustering of the total DEGs in axillary bud under N stress. FPKM values were scaled per gene across basal part samples and shown as the scaled expression. Ab stands for axillary bud. The number of genes in each cluster is indicated beside the cluster name. c Enriched Gene Ontology (GO) terms within the category of biological process for the ten clusters shown in (b). Only significant GO terms (false discovery rate (FDR) < 0.05) are displayed