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. 2020 May 8;27:61. doi: 10.1186/s12929-020-00656-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — INT777improved BBB permeability and TGR5 interacted with BRCA1 after MCAO. a INT777 treatment significantly reduced EB dye leakage, n = 6 per group; b INT777 alleviated the TEER decrease after OGD/R, n = 6 per group; c Double immunofluorescence staining showed that co-localization of TGR5 (red) and BRCA1 (green) was increased in penumbra 24 h after MCAO, d Triple-fluorescence staining showed that TGR5 and BRCA1colocalized in endothelial cell, n = 4 per group. Scale bar 50 μm. e Representative co-IP bands showed that interactions of TGR5 with BRCA1 occurred at 24 h after MCAO, n = 6 per group. f Expression of TGR5 and BRCA1 in total protein solution was detected by Western blot analysis and relative OD ratios were reported. n = 6 for each group. *P < 0.05 vs sham, ^#P < 0.05 vs MCAO+ vehicle. Bars represent mean ± SEM