Figure 4.

Detection of mutant K-ras in DNA from colonic ACF and tumor tissues. Examples of electropherograms indicating point mutations within K-ras gene amplified from large intestinal adenomas and carcinomas of F344 rats treated with AOM and fed 1% or 2% dietary phytate. (A) Identification of point mutations in codons 12 and 13 (exons 1 and 2) of the K-ras gene. (a) Normal K-ras codon 12 (GGT) and codon 13 (GGC); (b) adenoma with mutated codon 13 (GGC>CGC); (c) carcinoma with mutated codon 12 (GGT>GAT). AOM: Azoxymethane