Figure 2.

Depiction of age-related methylation at the CpG site chr6.150040098 annotated to large tumor suppressor kinase 1 (LATS1) and associated gene expression changes in the discovery and replication sample, respectively. (A) methylation difference between young and old (i.e. long-lived, ≥91 years) individuals in the discovery sample (extreme group comparison); (B) methylation changes over the age range 26–102 years in the replication sample; (C) positive correlation between methylation and gene expression; (D) gene expression difference between young and old (i.e. long-lived, ≥91 years) individuals in the discovery sample (extreme group comparison); (E) allele-specific methylation at rs10872646 with additive coding (0, homozygous for the major allele; 1, heterozygous; 2, homozygous for the minor allele).