Skip to main content
. 2020 Mar 11;29(7):1180–1191. doi: 10.1093/hmg/ddaa039

Figure 3.

Figure 3

Higher oxidative stress levels in mDANs compared with CPNs. (A) Cytosolic ROS and (B) mitochondrial superoxide were measured in Ctrl and PD Dupl CPNs and mDANs by the CellRox reagent and MitoSox, respectively, using a CLARIOStar Plus plate reader. CellRox and MitoSox signals were normalized to respective DAPI signals followed by the normalization to an external CPN and mDAN control line. Significantly higher ROS levels both (A) cytosolic and (B) mitochondrial were determined in PD Dupl mDANs compared with PD Dupl CPNs. (C) Total protein nitration level was determined in CPNs and mDNAs generated from Ctrl and PD Dupl iPSCs by WB using an antibody against nitrotyrosine. The blots were stained by Ponceau for protein loading control prior to immunodetection. Protein nitration levels are remarkably higher in mDANs compared with CPNs. Blots with samples from two independent differentiation experiments (#1 and #2) are shown. Blots for nitration within one black frame are derived from the same membrane. Ponceau staining was performed on the same membrane prior to the nitration detection. Lanes from different parts of the same membrane are separated by black dashed lines.