Figure 4.

Effect of DCEQA on the mRNA and protein expression levels of the MMP-1, MMP-2, MMP-9, and type Iα1 procollagen in UVB-irradiated HaCaT keratinocytes after 24 h from treatment analyzed by RT-PCR and Western blotting, respectively. Expression levels were quantified using the density of the bands and normalized against the levels of housekeeping gene β-actin. The effect of DCEQA on expression levels was given as the relative percentage of the UVB-irradiated (15 mJ/cm²) untreated control cells. Values are mean ± SD of three independent experiments. ^a–fMeans with different letters are significantly different at p < 0.05 level.