Table 1.

Apparent Michaelis—Menten (M-M) Kinetic Parameters for 21mer H3K4me3 Peptides in the Presence of Saturating (20 μM) Effector Peptide (Unmodified H3 10mer, aa 1–10)^a

21mer H3K4me3	${\mathit{K}}_{\mathbf{M}}^{\mathbf{app}}$ (× 10−6 M)	kcat (s−1)	kcat/${\mathit{K}}_{\mathbf{M}}^{\mathbf{app}}$ (M−1 s−1)
WT	12.5 ± 0.9	0.052 ± 0.001	4126.4 ± 300.6
R2A	153.6 ± 11.6	0.069 ± 0.002	451.8 ± 35.7
T3A	9.0 ± 0.6	0.056 ± 0.001	6232.4 ± 413.9
Q5A	415.2 ± 71.4	0.056 ± 0.003	134.4 ± 24.4
T6A	96.5 ± 15.7	0.075 ± 0.004	772.7 ± 131.6
R8A	55.1 ± 7.6	0.054 ± 0.002	980.7 ± 142.5
K9A	21.9 ± 2.8	0.061 ± 0.002	2791.3 ± 373.5
T6S	14.7 ± 1.5	0.070 ± 0.002	4756.4 ± 507.0
T6V	39.4 ± 6.8	0.050 ± 0.003	1258.5 ± 228.5

^aResults are means ± SEM of two independent experiments.