Table 2.

Apparent Michaelis—Menten (M-M) Kinetic Parameters for H3K4me3 WT and Mutant Peptides of Various Lengths^a

H3K4me3	${\mathit{K}}_{\mathbf{M}}^{\mathbf{app}}$ (× 10−6 M)	kcat (s−1)	kcat/${\mathit{K}}_{\mathbf{M}}^{\mathbf{app}}$ (M−1 s−1)
21mer	24.9 ± 3.3	0.057 ± 0.002	2289.3 ± 312.3
18mer	62.3 ± 5.6	0.078 ± 0.002	1248.8 ± 117.2
13mer	488.7 ± 107.5	0.111 ± 0.009	227.6 ± 53.7
18mer-K14A/R17A/K18A	514.1 ± 87.6	0.108 ± 0.007	210.9 ± 38.6
18mer-K14acK18ac	249.1 ± 28.5	0.101 ± 0.004	404.6 ± 48.7

^aResults are means ± SEM of two independent experiments.