Figure 1. GA ₅₀ aggregates are detected in neurites of cortical neurons over time.

Primary rat cortical neurons transfected with eGFP‐GA₅₀ were examined to determine at which time points preceding cell‐death aggregates are found in neurites. Two days post‐transfection, aggregates formed by eGFP‐GA₅₀ (green) are detectable in neurites (SMI‐32 staining in red). These aggregates remain localized to neurites at 96 h (4 days) and 288 h (8 days). Colocalization is indicated by yellow overlay of colors (right panels). Inset below each image shows enlargement of neurite regions containing aggregates, representative fields from 60× magnification z‐stack confocal images, scale bar indicates 20 μm. Inset scale bars indicate 5 μm.