Graphical representation is the change in fluorescence (ΔF) normalized to basal fluorescence (F), ΔF/F. The final 10 s of baseline recording and 10 s of the depolarization phase are shown; arrow indicates start of depolarization. Non‐linear regression was performed in GraphPad Prism7 using the plateau followed by one‐phase decay model. The decay constant (k) for eGFP was 0.4971 s
−1, while for GA
50, it was 219.5 s
−1. Similarly, the tau (τ) values were 2.012 s for eGFP and 0.00455 s for GA
50, respectively. Data presented as mean ± SEM for each imaged timepoint. Statistical significance was determined using the Sidak–Bonferroni method, ****
P < 0.0001,
n = 20 puncta regions from four biological replicates. Exact
P‐values can be found in
Appendix Table S1.