Figure 9. SV2 is specifically reduced in spinal cord lysates of symptomatic transgenic GA149‐CFP mice.

Spinal cord lysates were generated from fresh‐frozen tissue of 20‐month‐old mice either expressing GA₁₄₉‐CFP under the Thy1 promoter (Tgx GA₁₄₉) or wild‐type controls.

• A–C
  Lysates were immunoblotted for SV2 (A), synaptophysin (B), and PSD‐95 (C). Total protein levels were determined by imaging the Mini‐PROTEAN TGX stain‐free gel prior to transfer. Quantification of band intensities normalized to total protein loading revealed a significant reduction in SV2 protein levels in the GA‐expressing cohort compared with wild‐type animals, while synaptophysin and PSD‐95 levels remained unaltered. Data presented as mean ± SEM. Unpaired t‐test, *P < 0.05. A total of four animals per group were examined. Exact P‐value can be found in Appendix Table S1.

Source data are available online for this figure.