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Effect of increasing [EtNH3+] on v/[E] (s–1) for K120A/Q295A variant hlGPDH-catalyzed reduction of DHAP by NADH for reactions at pH 7.5 (20 mM TEA buffer), 25 °C, saturating [NADH] = 0.2 mM, and I = 0.12 (NaCl): (●) 80 mM cation, (■) 60 mM cation, (▼) 40 mM cation, (▲) 20 mM cation, and (◆) 10 mM cation. The inset shows the plot of (kcat/Km)obs against [EtNH3+], where (kcat/Km)obs values are the slopes of the correlations from the main panel.
