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. 2020 May 8;15(5):e0232689. doi: 10.1371/journal.pone.0232689

Table 3. pH and volatile fatty acid concentrations in faeces for the two groups across the 11 study days.

Study day SED Benjamini-Hochberg P-value
Group 1 2 3 4 5 6 7 8 9 10 11 Day Group D*G Day Group D*G
Acetate (mM) C 10.35 12.21 13.33 12.10 10.81 11.18 11.19 11.92 15.20 11.84 11.48 1.07 1.50 1.81 0.03 0.87 0.77
A 12.26 12.00 12.10 12.13 8.88 10.05 11.22 12.33 12.54 14.12 11.41
Butyrate (mM) C 1.17 1.19 1.28 1.19 1.01 1.11 1.06 1.33 1.51 1.11 1.11 0.15 0.18 0.24 0.06 0.64 0.58
A 1.41 1.32 1.43 1.26 0.96 1.09 1.20 1.36 1.47 1.65 1.29
Propionate (mM) C 3.28 3.45 3.77 3.42 2.87 2.99 2.71 3.02 3.60 2.95 3.19 0.34 0.72 0.68 0.00 0.64 0.58
A 3.80 4.21 4.34 3.56 2.59 2.95 3.16 3.85 4.16 4.90 3.74
BCVFA (mM) C 1.22 1.51 1.32 1.11 1.07 1.21 1.26 1.38 1.69 1.10 1.06 0.41 0.33 0.62 0.63 0.57 0.58
A 1.73 1.56 1.56 3.38 1.61 1.40 1.60 1.44 1.51 1.84 1.39
pH C 6.32 6.43 6.32 6.43 6.40 6.44 6.25 6.17 6.16 6.12 6.35 0.06 0.08 0.10 0.00 0.57 0.58
A 6.50 6.36 6.34 6.56 6.42 6.48 6.40 6.38 6.32 6.29 6.42

Faecal pH and VFA concentrations for samples collected over the 11 study days (final 3 days hay feeding and 8 days hay plus barley feeding) between the two groups (C, Control; A, Aged). BCVFA = Branched chain volatile fatty acids. SED: standard error of the difference.