Tests of cancerous K-562 cells at a free-field pressure of 0.7 MPa, pulse duration of 100 ms and duty cycle of 10%, showing the effect of frequency and number of cycles. (a) Cell death versus number of cycles and (b) cell death at 1.8 million cycles. Unpublished data from DR Mittelstein, J Yian, EF Schibber, A Roychoudhury, LT Martinez, MH Fekrazad, M Ortiz, PP Lee, MG Shapiro, M Gharib (2020) [16]. (Online version in colour.)