Table 1. Mycobacterium tuberculosis gene regions targeted for detection of drug resistance associated mutations and associated primers.

Drug	Gene	Segment Name	Targeted Nucleotide Positions	Primer Direction	Primer Sequence (5’ to 3’)
Rifampicin	rpoB	rpoB	1215 to 1383	Forward	GATCACACCGCAGACGTTGA
				Reverse	ACGCTCACGTGACAGACCG
Isoniazid	inhA promoter	inhA promoter	nucleotide -160 to 44 of mabA gene	Forward	CCCAGAAAGGGATCCGTCAT
				Reverse	GATACGAATGGGGGTTTGGC
	katG	katG	809 to 992	Forward	CTGTTGTCCCATTTCGTCGG
				Reverse	GGCGGTCACACTTTCGGTAA
Ethambutol	embB	emb10	841 to 1017	Forward	GCCGTGGTGATATTCGGCTT
				Reverse	CAGCGCCAGCAGGTTGTAAT
		emb20	1156 to 1299	Forward	GCGGCGGCCATGGTCTTG
				Reverse	CAGCGCCGCCGGTGTGA
		emb40	1451 to 1550	Forward	CCGCCGGCACCGTCATCCTGA
				Reverse	GCCTGGCTCGGCCCGATTTTG
Fluoroquinolones/ Moxifloxacin/ Ofloxacin	gyrA	gyrA	159 to 395	Forward	CCGGGTGCTCTATGCAATGT
				Reverse	GCTTCGGTGTACCTCATCGC
Fluoroquinolones	gyrB	gyrB	1390 to 1639	Forward	GAGTTGGTGCGGCGTAAGAG
				Reverse	CCGTGATGATCGCCTGAACT
Kanamycin	eis	eis promoter	-81 to 34	Forward	CGTCCTCGGTCGGGCTACACAG
				Reverse	GCATCGCGTGATCCTTTGCCAGAC
Streptomycin	rpsL	rpsL0	54 to 170	Forward	GGTCAAGACCGCGGCTCTGA
				Reverse	AACTTCACGCGGGCAACCTTC
		rpsL1	183 to +53	Forward	CGAGGTCACGGCGTACATTC
				Reverse	GTAGACCGGGTCGTTGACCA
	rrs	rrs10	465 to 689	Forward	TCGGATTGACGGTAGGTGGA
				Reverse	CATTCCACCGCTACACCAGG
Streptomycin /Amikacin/Capreomycin/Kanamycin		rrs30	1368 to +59	Forward	ATACGTTCCCGGGCCTTGTA
				Reverse	AGACAAGAACCCCTCACGGC
Pyrazinamide	pncA	pncA3	-69 to 154	Forward	CAACAGTTCATCCCGGTTCG
				Reverse	TCGGTATTGCCACCGATCAT
		pncA2	111 to 337	Forward	CCAAGCCATTGCGTACCG
				Reverse	ATCCCAGTCTGGACACGTCG
		pncA1	245 to 454	Forward	CGTTCTCGTCGACTCCTTCG
				Reverse	AGCGGCGGACTACCATCAC
		pncA0	392 to +29	Forward	TGTGGAAGTCCTTGGTTGCC
				Reverse	CCCTATATCTGTGGCTGCCG