Fig 1. Study design.

Left lung from C57BL/10 inbred mice were orthotopically transplanted into minor histocompatibility antigen mismatched C57BL/6 mice. Both inbred mice were used for sham. The transplanted lung grafts were harvested and classified into OB or non-OB by pathological examination at 21th day post transplantation. The left lungs of shams were also harvested at the same time. Three samples in each group (total 9 lungs) were subjected individually to microarray analysis. After representative OB marker genes were extracted from microarray analysis, real-time quantitative PCR were performed for lungs, left mediastinal lymph nodes, and spleens using at least 5 animals for each group.