Figure 2.

(a) Sequences selected based on their frequency and reproducibility (P1, P2, and P3-peptides) were enriched over the rounds of screening. The negative control selected from the naïve library did not withstand the selection pressure and decreased in relative frequency with successive rounds of selection. R0: Original naïve library, P: Peptides selected from phage display. Data represents mean ± SD of two replicates of library screening. Multiple sequence alignment of the thirty most frequent sequences from (b) replicate 1 and (c) replicate 2 of the fourth round of phage screening visualized using Seq2Logo generator to select synthetic motifs P4-peptide and P5-peptide, respectively.