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. 2020 May 4;375(1801):20190403. doi: 10.1098/rstb.2019.0403

Figure 4.

Figure 4.

Anthocyanins do not control PHANG expression. (a) Quantification of anthocyanins in seedlings grown for 5 d in continuous light in the presence of NF. WT, gun1-1, gun5-1, ref3-3 (point mutant for C4H) and double mutants were analysed. Data are given as mean ± s.d. (n = 4). Letters indicate statistical groups determined by Student's t-test (p < 0.05). FW, fresh weight. (b,c) qRT-PCR analysis of gene expression for genes encoding PHANGs (CARBONIC ANHYDRASE1 and PLASTOCYANIN). Data are given as mean ± s.d. (n = 3). Letters indicate statistical groups determined by Student's t-test (p < 0.05). (d) Representative photograph of 5 d-old WT (Col-0 and Nos), transparent testa (tt) 6, tt18, the dominant pap1-D overexpression line and myb75 mutant grown in continuous light in the presence of norflurazon. (e) qRT-PCR analysis of gene expression for LIGHT HARVESTING COMPLEX B1.2 in genotypes shown in (d). (f) qRT-PCR analysis of CHS, DFR, LDOX and MYB75 expression in pap1-D and myb75 mutant grown in the presence of NF. For the analysis shown in (b,c,e,f) the expression was calculated relative to the NF-treated WT and ACTIN2 as reference (ΔΔC(t)method). Data are given as mean ± s.d. (n = 3). The Nossen ecotype was used as control for the myb75 mutant (*).