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. 2020 May 8;6(19):eaaz2166. doi: 10.1126/sciadv.aaz2166

Fig. 2. The excitability of burs neurons regulates wing expansion and sleep.

Fig. 2

(A) burs-GAL4/+>UAS-GFP/+ labels two neurons in the SEG (Bseg) and 12 to 14 neurons in the abdominal ganglion (Bag). Scale bar, 20 μm. (B) burs-GAL4/+>UAS-Kir2.1/+ flies slept more than parental controls (n = 20 to 27 flies per genotype; repeated-measures ANOVA for Time × Genotype, P < 0.001). (C and D) burs-GAL4/+>UAS-Kir2.1/+ flies displayed increased daytime sleep and sleep bout duration compared to controls (*P < 0.01, Tukey correction). (E) Bseg>UAS-Kir2.1 flies with unexpanded wings slept more than parental controls (n = 16 to 32 flies per genotype; repeated-measures ANOVA for Time × Genotype, P < 0.001). (F and G) Bseg>UAS-Kir2.1 had more daytime sleep, but sleep consolidation was not altered (*P < 0.01, Tukey correction). (H) Bag>UAS-Kir2.1 flies had normal wings and modestly increased sleep (n = 18 to 30 flies per genotype; repeated-measures ANOVA for Time × Genotype, P < 0.001). (I and J) Bag>UAS-Kir2.1 flies modestly increased daytime sleep without altering sleep consolidation (*P < 0.01, Tukey correction).