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. 2020 May 4;13:383–393. doi: 10.2147/PRBM.S250945

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© 2020 Yisireyili et al.

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Stress induced expression of gastric monocyte/macrophage markers in mice. The immunohistochemistry and RT-PCR method were used to analyze the immunostaining and mRNA expression levels of CD11b, CD68, and F4/80 in the stomach of mice in the stress and control groups. The values for the stressed mice are presented in comparison with those of the control mice and are expressed as mean±SD (n=15). Student’s t-test was performed to test the differences between the stress and control groups. (A) CD11b-positive cells (monocytes), (B) CD68, and (C) F4/80 in the stomach tissue of both control and stressed mice (200× magnification, bar=50 µm); (D) quantitative analysis of CD11b-positive cells relative to the total number of nuclei. **P<0.001 compared with the control mice; (E) quantitative analysis of CD68 mRNA and (F) F4/80 mRNA expression levels in stomach tissue. **P<0.001 compared with the control mice.