Figure 5.

In vivo antitumor activity of CD44-CAR T cells against HCC. (A) HCC xenograft mice were given 1×10⁷ effector cells treatment. After mice were sacrificed, tumor tissues of CD44-CAR T cells treated mice were harvested. Immunofluorescence images showed different CD44 expression and T cells infiltration (anti-CD3 staining) in five types of HCC cells. Scale bar = 50 μm. (B) After the tumor volume of xenograft mice reached about 100mm³, effector cells were injected twice (arrows marked). The volumes of xenograft mice tumors were recorded for statistical analysis. (C) One hundred μL of peripheral blood from treated mice on day 7 were obtained for the in vivo cytokine secretion assay according to the ELISA kit instrument. All statistics are presented as the means ± SDs, n = 5 for each group, ** P<0.05 vs normal T and mock T groups.