Figure 1. Multi-area two-photon imaging across S1 and S2 during a delayed non-match-to-sample task.

(A) Schematic of behavioral task. (B) Behavior performance from two-photon imaging experiments. Overall performance across recording sessions are shown along with performance for each trial condition. Performance for individual animals (open circles) are shown along with the mean for all animals (black circle). Error bars: s.e.m. (C) Schematic of multi-area imaging experiment including; viral injection scheme for calcium indicator expression and labeling of projection neurons (upper left); example in vivo two-photon images of expression in S2 and S2 (upper right). Scale bar: = 100μm; head-fixed mouse under multi-area two-photon microscope and behavioral rig (bottom). (D) Example simultaneous recordings from S1 and S2 neurons during behavioral task along with extracted periods of whisker-to-rotor touch (gray area), whisker angle, and whisker curvature from high speed videography.