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. 2020 May 8;3:225. doi: 10.1038/s42003-020-0960-y

Fig. 5. Increased permeability to Evans Blue dye in the NTS at early night.

Fig. 5

a Representative images of the rostral-caudal DVC showing the merge of anti-vimentin and anti-GFAP staining (top row) for identification of the AP/NTS glial border along with annotations of the ROIs of the NTS taken for subsequent analysis. Evans Blue dye (EB) fluorescence imaging (middle & bottom rows) with added annotation of the AP/NTS border at ZT1 vs ZT13; note the increased penetration of EB into the adjacent NTS at ZT13. White bar depicts 100 µm. b Intensity of EB staining in the whole, medial and lateral areas of the NTS for the intermediate level sample slice during LD (top panel) or DD (bottom panel) (*p < 0.05, **p < 0.01, t tests or Mann–Whitney tests). c Gene expression relative to CT of the tight junction proteins Claudin-5 (Cldn-5) and Zona occludens-1 (Tjp-1) in the AP/glial border over 24 h (*p < 0.05, one-way ANOVAs). L lateral, M medial.