Fig. 3. SMSN reconstruction of immune-labeled Nup98 protein in COS-7 cells.

a Lateral view of SMSN reconstruction from the PR-4Pi method. b 700-nm thick x–y slice of selected region in a (white dash box). c 300-nm thick x–z slices of selected regions in b (between white dash lines). d, e Selected single nuclear pore complexes (NPC) in the x–y plane and y–z plane and their profiles along the central line, obtained by averaging the pixel values within the white box along the orthogonal dimension of the central line. Each peak was fitted by a 1D Gaussian with a width of $\sigma$, and the FWHM was calculated from 2.355$\sigma$. One column as indicated by the red dash box shows the same NPC in its lateral and axial views. The sample was imaged at a single-optical section with 90,000 frames under epi illumination. Scale bar: 2 µm in a, 200 nm in inlets of a, 500 nm in b, c, 100 nm in d, e.