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. 2020 Apr;8(7):459. doi: 10.21037/atm.2020.03.108

Figure 2.

Figure 2

The function domain of Sam68 mediates aerobic glycolysis. (A) Schematic diagram for Sam68-fusion protein (residues 1–443), full-length or designated mutants (ΔN lacks residues 1–102, ΔC lacks 347–443, and ΔKH lacks 162–228) fused with HA tag. (B,C) The pyruvate kinase activity and lactate production were measured in CRC cells transfected with different Sam68 mutants. (D) Schematic diagram of Sam68 mutation (G178E) in the KH domain. (E,F) The effects of Sam68 G178E mutation on the pyruvate kinase activity and lactate production in CRC cells. *P<0.05, ***P<0.001. CRC, colorectal cancer.