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. 2020 May 8;10:7822. doi: 10.1038/s41598-020-64700-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Liver transcription program is expressed in HepaRG cells at one week of differentiation. (A) HepaRG differentiation model. For proliferative (progenitor) condition, cells were seeded and trypsinized before reaching 50% confluence; for differentiating conditions, cells were seeded at 70–80% confluence in order to reach 100% confluence 24 h after seeding. (B) Transcriptome was analyzed in both conditions. Heatmap represents differentially expressed genes (DEGs) with fold change greater than four. Cell/tissues types associated with genes down-regulated (C) and up-regulated (D) in differentiating cells (EnrichR), adjusted p-values are shown. (E–H) Expression of hepatocyte markers was validated by RT-qPCR, data represent mean ± SEM 3 independent cultures/condition; *Statistical difference (p < 0.05).