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. 2020 May 8;10:7750. doi: 10.1038/s41598-020-64696-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vector competence of sylvatic Ae. malayensis and Ae. aegypti controls after exposure to 1.16–1.38 ×10⁷ FFUs/ml of DENV-1. Bars represent the percentage of virus-positive mosquitoes 14 days post infectious blood meal and the error bars are the 95% confidence intervals of the percentages. Infection rate (IR) is the proportion of blood-fed females with an infected body. Dissemination rate (DR) is the proportion of infected females with virus disseminated to the head tissues. Transmission rate (TR) is the proportion of females with a disseminated infection that shed virus in their saliva. Transmission efficiency (TE) is the overall proportion of blood-fed females that shed virus in their saliva. The Ae. aegypti population was included as a positive control. The figure compiles data from two independent experiments that did not differ significantly. Blood meal titers were 1.16 ×10⁷ and 1.38 ×10⁷ FFUs/ml in the first and second experiment, respectively. **p < 0.01; ***p < 0.001.