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. 2020 May 8;10:7752. doi: 10.1038/s41598-020-64831-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Expression of Trk receptors assessed using flow cytometry on cultures after 7 days of exposure to neurotrophic factors (day 15 of differentiation). Unstained neurons were used as negative control (light blue histograms). In each panel, the average of positive cells observed for each marker in all trials performed (n = 4) is reported: TrkA⁺ neurons represented the 1–2% of differentiated cultures (violet histogram), TrkB⁺ the 25–20% (green histogram), while TrkC⁺ cells were the 60–70% (red histogram) in analyzed samples. Channels used for detection included: DAPI (Alexa Fluor 405), FITC (Alexa Fluor 488), PE (Phycoerythrin). (B) Fold-difference of Trk receptor and proprioceptor marker expression by quantitative RT-PCR analysis in sorted cells vs. starting sensory neurons cultures.