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. 2020 May 8;10:7794. doi: 10.1038/s41598-020-64777-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Photos and graphics showing the results of overexpression of WFhb1-1 in near-isogenic lines Qfhb1-NIL-S (A,B) and Qfhb1-NIL-R (C,D) or silencing of WFhb1-1 in FHB-resistant cultivar Sumai 3 (E,F) and FHB-susceptible landrace Y1193-06 (E) using a barley-stripe-mosaic virus (BSMV)-based system. Viral inoculation on spikes was applied. dpfi: days post Fusarium inoculation; FES: viral inoculation buffer FES; BSMV:00: wildtype BSMV; BSMV:W: WFhb1-1-overexpressing BSMV; BSMV:T: WFhb1-1-silencing BSMV; _L leaf inoculation of BSMV; _S: spike-inoculation of BSMV. The red or black dots on spikelets indicate the inoculated spikelets.