Fig. 2. Drosophila accessory gland is subjected to oncogene-dependent epithelial cells division and extrusion.

(a, b) and (d, e) Green fluorescence reveals clones of GFP expressing cells (WT). a–c Clonal cells expressing GFP/nlsGFP only. d–e Clonal cells co-expressing GFP and Ras^V12 (Ras^V12). a, b Clonal induction is revealed by multifocal localisation of GFP expressing cells (white arrows). Due to time of induction, clones in accessory gland epithelium are composed of two neighbouring cells. These cells do not display phenotype of hypertrophy, as quantified in (c) (Mann–Witney test; P = 0,3431). Data are represented as mean values ± SEM (n = 8 and n = 12 accessory glands Control and GFP analysed respectively, from two experiments). d, e GFP-Ras^V12 expressing clones are composed of numerous cells and are present outside the gland. DAPI (blue) reveals nuclei in (a, b) and (e). Representative images in (a–e) from three or more experiments. Scale bars: 200 μm in (a) and (d), 50 μm in (b, e).