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. 2020 May 8;11:2300. doi: 10.1038/s41467-020-16123-w

Fig. 7. RasV12-driven tumour formation depends on the setting of an EGF/EFGR autocrine activation loop.

Fig. 7

a, b Immunostaining reveals that EGF/Spitz (Spitz) is expressed in GFP-RasV12 expressing clones. c Invasive tumour frequency of GFP-RasV12 expressing clones (RasV12) is impaired by downregulation of EGF/Spitz (Spitz RNAi) (Chi2 test; P < 0.0001). d Clonal expression of a secreted form of EGF/Spitz (Spisc) leads to tumour formation. e Invasive tumour frequency of GFP-RasV12 expressing clones (RasV12) is impaired by downregulation of EGFR (EGFR RNAi) (Chi2 test; P < 0.0001). f Clonal expression of a constitutively active form of EGFR (EGFRƛ) induces the formation of tumours. g Invasive tumour frequency of GFP- EGFRƛ expressing clones (EGFRƛ) is strongly impaired by downregulation of Ras (Ras RNAi) (Chi2 test; P < 0.0001). Data are represented as mean values±SEM. Representative images in (a, b, d, f) from three or more experiments. ****P < 0.0001. Scale bars: 50 μm.