Figure 3.

CAR^LunX T Cells Are Toxic against LunX-Positive Lung Cancer Cells

(A) Immunofluorescent staining for LUNX in NSCLC (NCI-H292, NCI-H1650, A549, and NCI-H358) and lung fibroblasts (HFL1). Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar, 50 μm. (B) Toxicity of LunX-based CAR T cells against the lung cancer cell lines NCI-H292, NCI-H1650, and A549 at a ratio of effector cell:target cell of 10:1. The control indicates that effector cells were not added. One of three independent experiments is shown. (C) Toxicity of CAR^LunX T cells and CAR^CD19 T cells against the lung cancer cell lines NCI-H292, NCI-H1650, and A549 at different ratios of effector cell:target cell for 40 h. One of three independent experiments is shown.