Figure 4.

Recognition of LunX-Positive Lung Cancer Cells Induces CAR^LunX T Cells to Secrete Cytokines and Proliferate

(A) CAR^LunX T cells and CAR^CD19 T cells were incubated with LunX-positive lung cancer lines NCI-H292, NCI-H1650, and A549 for 24 h. Then, the supernatant was harvested and expression of IFN-γ. (B and C) (B) IL-2, and (C) TNF-α was measured by ELISAs. n = 3, results are representative of three independent experiments. Data in (A)–(C) are the mean ± SEM. Unpaired t test, ∗∗∗∗p < 0.0001. (D) Proliferation assays undertaken on CAR^LunX T cells and CAR^CD19 T cells using cell trace violet staining following in vitro incubation with the LunX-positive cancer cell lines NCI-H292, NCI-H1650, and A549 demonstrated antigen-specific proliferation of CAR^LunX T cells. Experiments were repeated twice independently. ∗∗p < 0.01.