Fig. 5.

Validation of alternative splicing events of hnRNPA1. (A) Sashimi plots show AS events occurred in hnRNPA1. Reads density for alternative exon 8 were illustrated. Multiple transcripts for the gene are shown below. (B) Detection of exon 8 skipping of hnRNPA1. The schematic diagram depicts the skipping of exon 8 in hnRNPA1. The exons are denoted by black or red boxes and intron by horizontal line. RNA-seq quantification (left, exon skipping ratios) and RT-qPCR validation (right) of the AS events were shown below (**P < 0.01; *P < 0.05; t-test). (C) Validation of alternative splice junctions in hnRNPA1. Two splice junctions result in exon 8 inclusion or exclusion were shown. (D) Western blot analysis of hnRNPA1 protein isoforms. The two isoforms of hnRNPA1 were illustrated. Tubulin is a loading control.