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. 2020 Jan 17;10(1):23–42. doi: 10.1016/j.jcmgh.2020.01.005

Figure 5.

Figure 5

ATF2 and ATF7 are required for appropriate epithelial repair upon damage. (A) Villin-CreERT2-Atf2wt/wtAtf7+/+ (n = 11) and Villin-CreERT2-Atf2fl/flAtf7ko/ko (n = 11) were injected intraperitoneally with 1 mg tamoxifen for 5 consecutive days. Fourteen days after induction of recombination mice were irradiated with 12 Gy and killed (†) 96 hours after irradiation. (B) Representative images of H&E immunohistochemistry of irradiated colons 96 hours after irradiation. Scale bars: 50 μm. (C) Representative images of immunohistochemistry for BrdU in colons of irradiated mutant and control animals. Scale bars: 50 μm. (D) Quantification of BrdU-positive cells in colonic crypts. n = 11 mice per group. (E) Representative images of scratch wound assay of Atf2wt/wtAtf7+/+ (left panels) and Atf2-/-Atf7ko/ko (right panels) cell monolayers at 0 hours (left panels) and 16 hours (right panels) after scratching. Wounds artificially have been colored black and the scratch edges colored red to aid visualization. (F) Representative experiment showing SEM based on measuring the area of 8 fields of the wound per condition. The experiment was repeated 3 independent times with similar results. The area of the wound (shown in panel E) covered by Atf2fl/flAtf7+/+ (black circles) and Atf2-/-Atf7ko/ko (red circles) was measured every 2 hours and quantified with ImageJ. n = 3 independent experiments. Graph bars show means and SEM. ****P < .0001, Student t test. HE, hematoxylin-eosin.