Table 2.
Primers Used for qRT-PCR Experiments
| Gene | Forward primer | Reverse primer |
|---|---|---|
| Actin | GATGCTCCCCGGGCTGTATT | GGGGTACTTCAGGGTCAGGA |
| AlpI | TGGTCACAGCAGTTGGTAGC | TGACGTTCCGATCCTGAGTG |
| Ascl2 | GAAGGTGCAAACGTCCACTT | TCCATCAAGCTTGCATTCAG |
| Atf2 | GACTCCAACGCCAACAAGAT | AGGTAAAGGGCTGTCCTGGT |
| Atf2 (floxed exon) | CCAGCTCACACAACTCCTCA | GGACTGAACCCACACTTTCC |
| Atf7 | AGACAGATTGTGGGACCGAG | CACAAACGGTCTGTCGTCTC |
| Atf7 (floxed exon) | CGGCGTACAGTGGATGAAGAT | CATTGCTAGGGGCTGATGCG |
| Cd44 | TCTGCCATCTAGCACTAAGAGC | GTCTGGGTATTGAAAGGTGTAGC |
| Cdh1 | GAGGTCTACACCTTCCCGGT | CCACTTTGAATCGGGAGTCT |
| ChgA | GTCTCCAGACACTCAGGGCT | ATGACAAAAGGGGACACCAA |
| Cldn15 | GCTTCTTCATGTCAGCCCTG | TTCTTGGAGAGATCCATGTTGC |
| Cldn2 | CCAGCCTCCAAGGGTTTCAT | TCTAGAAAACGGAGCCGTCC |
| Lgr5 | TGTGTCAAAGCATTTCCAGC | CAGCGTCTTCACCTCCTACC |
| Lyz1 | GGATGGCTACCGTGGTGTCAAGC | TCCCATAGTCGGTGCTTCGGTC |
| Muc2 | GAAGCCAGATCCCGAAACCA | GAATCGGTAGACATCGCCGT |
| Olfm4 | AACATCACCCCAGGCTACAG | TGTCCACAGACCCAGTGAAA |
| Sis | AGGTGTCCGCCTGAGCAAGGT | ATGGACGCCAGCAACAGCCA |
| Vil1 | ATCTCCCTGAGGGTGTGGAC | AGAGAAGGCAGCTGGAGTCA |
| Zo1 | AGGACACCAAAGCATGTGAG | GGCATTCCTGCTGGTTACA |