Fig. 6A.

SDS-PAGE gel (4–20%) of non-denatured Avidin (n-Av) and denatured Avidin (d-Av) stained with Coomassie Brilliant Blue R-250. B. Native PAGE gel (7.5%) of Avidin (Av), PEG, 1:2 mAv and 1:4 mAv under reverse polarity stained with (left) iodine for PEGs and with (right) Coomassie Brilliant Blue R-250 for protein. C. UPLC analysis of standard Avidin shows peak ‘a’ at 6.29 min. 1:6 mAv and 1:4 mAv formulation have similar peak shape showing a majority of mAv with 4 PEGs (peak ‘b’ at 4.38 min or 4.37 min) and a secondary population of mAv with 2 PEGs (peak ‘d’ at 5.33 min). In addition, 1:2 mAv has three peaks, a majority of mAv with 2 PEGs (peak ‘d’ at 5.35 min), a secondary population of mAv with 3 PEGs (peak ‘c’ at 4.80 min) and a minority of mAv with 1 PEGs (peak ‘e’ at 5.86 min).