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. 2020 Apr 6;37:100988. doi: 10.1016/j.molmet.2020.100988

Figure 3.

Figure 3

Inflammasome inhibitor treatment diminishes the accelerated insulin resistance development in O-HFD mice. (A) Glucose and insulin tolerance tests after 8 weeks of HFD. Values represent the mean ± SEM for 11 O-ND and 11 O-HFD mice treated with PBS, as well as 11 O-ND and 11 O-HFD mice treated with MCC950. ∗P < 0.05 and ∗∗P < 0.01 versus O-ND treated with PBS. #P < 0.05 and ##P < 0.01 versus O-HFD treated with PBS. O-ND, offspring of ND-fed dam; O-HFD, offspring of HFD-fed dam; GTT, glucose tolerance test; ITT, insulin tolerance test, AUC, area under the curve. (B) Representative images of a hematoxylin-and-eosin-stained eWAT from O-ND and O-HFD mice, respectively. The arrow shows CLS. Scale bar = 50 μm. O-ND, offspring of ND-fed dam; O-HFD, offspring of HFD-fed dam. (C) Quantitative analysis of adipocyte size and the number of CLSs. Values represent the mean ± SEM. Each group consisted of five mice. ∗P < 0.05 and ∗∗P < 0.01 versus O-ND treated with PBS. ##P < 0.01 versus O-HFD treated with PBS. O-ND, offspring of ND-fed dam; O-HFD, offspring of HFD-fed dam. (D) eWAT concentrations of IL-1β after 8 weeks of HCD with or without MCC950. Values are the mean ± SEM for 10 O-ND and 11 O-HFD mice treated with PBS, as well as 10 O-ND and 11 O-HFD mice treated with MCC950. Data were expressed as protein concentration levels relative to those of O-ND treated with PBS. ∗∗P < 0.01 versus O-ND treated with PBS. ##P < 0.01 versus O-HFD treated with PBS. O-ND, offspring of ND-fed dam; O-HFD, offspring of HFD-fed dam.