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. 2020 Apr 6;37:100988. doi: 10.1016/j.molmet.2020.100988

Figure 6.

Figure 6

Caspase-11 activity is enhanced in BMDMs of O-HFD. (A and B) Representative western blot of caspase-11 of BMDMs before and after LPS priming as well as quantitative analysis of protein expressions. Values are the mean ± SE for 3 O-ND and 3 O-HFD mice before LPS priming, as well as 3 O-ND and 3 O-HFD mice after LPS priming. ∗∗P < 0.01 versus O-ND (before LPS priming). ##P < 0.01 versus O-HFD (before LPS priming). P < 0.05 versus O-ND (after LPS priming). #P < 0.05 (O-ND versus O-HFD). LPS, lipopolysaccharide; M0, BMDMs before LPS priming; O-ND, offspring of ND-fed dam; O-HFD, offspring of HFD-fed dam. (C and D) Flow cytometric analysis of the caspase-1 activity in BMDMs, as well as quantitative analysis of MFI, after 2 h of PA stimulation following LPS priming. Values are the mean ± SEM for 5 O-ND and 5 O-HFD mice. LPS, lipopolysaccharide; PA, palmitate; O-ND, offspring of ND-fed dam; O-HFD, offspring of HFD-fed dam. (E and F) Representative western blot of caspase-1 of BMDMs before and after LPS priming as well as quantitative analysis of protein expressions. Values are the mean ± SE for 3 O-ND and 3 O-HFD mice before LPS priming, as well as 3 O-ND and 3 O-HFD mice after LPS priming. LPS, lipopolysaccharide; M0, BMDMs before LPS priming; O-ND, offspring of ND-fed dam; O-HFD, offspring of HFD-fed dam. (G and H) Representative western blot of caspase-1 of BMDMs after 2 h of PA stimulation following LPS priming as well as quantitative analysis of protein expressions. Values are the mean ± SE for 3 O-ND and 3 O-HFD mice. PA, palmitate; LPS, lipopolysaccharide; O-ND, offspring of ND-fed dam; O-HFD, offspring of HFD-fed dam.