Mature IL-1β release is augmented in BMDMs of O-HFD after metabolic activation. Protein concentrations of IL-1β in the supernatant of BMDMs after metabolic activation. Values are the mean ± SE for 5 O-ND and 5 O-HFD mice without LPS priming, as well as 5 O-ND and 5 O-HFD mice with LPS priming. ∗∗P < 0.01 versus O-ND (PA). ##P < 0.01 versus O-HFD (PA). ¶P < 0.05 versus O-ND (LPS + PA). LPS, lipopolysaccharide; PA, palmitate; O-ND, offspring of ND-fed dam; O-HFD, offspring of HFD-fed dam.