Figure 5.
The modified SIB1 proteins do not complement the lsd1 sib1 mutant. A, Images were taken from continuous light-grown 20- and 24-d-old wild-type (WT), lsd1, and lsd1 sib1 (l/s) plants as well as transgenic lsd1 sib1 plants expressing the different variants of pSIB1-driven GFP-tagged SIB1. B, Cell death was visualized by TB staining. C and D, Ion leakage for the quantification of cell death (C) and Fv/Fm for chlorosis (D) were determined. First and second leaves from 24-d-old plants were harvested for measuring ion leakage and Fv/Fm. Six leaves per genotype were used for each measurement. Data represent means from three independent measurements, and error bars indicate sd. Lowercase letters indicate statistically significant differences between mean values at each of the indicated time points (P < 0.05, one-way ANOVA with posthoc Tukey’s HSD test). E, The relative transcript levels of immune-related genes in 20-d-old plants were analyzed using RT-qPCR. ACTIN2 (ACT2) was used as an internal standard. The data represent means of three independent biological replicates, and error bars indicate sd. Lowercase letters indicate statistically significant differences between mean values at each of the indicated time points (P < 0.05, one-way ANOVA with posthoc Tukey’s HSD test).