Figure 1.

Expression patterns of BLH2 and BLH4 in developing seeds. A, RT-qPCR analysis of BLH2 and BLH4 expression in siliques at different developmental stages from 4 to 13 DPA. The ACTIN2 gene was used as the internal control. The expression level at 4 DPA was set as 1. Data are means ± sd of three biological replicates. B, In situ hybridization showing the transcripts of BLH2 and BLH4 in seed coats at different developmental stages. Mu, mucilage; SG, starch granule; Co, Columella; RW, radial cell wall; CP, cytoplasm. Scale bars = 50 μm. The experiments were independently performed twice and similar results were obtained.