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letter
. 2020 Feb 27;183(1):37–40. doi: 10.1104/pp.20.00212

Figure 1.

Figure 1.

Hypocotyl gravitropic bending termination depends on TIR1/AFB signaling. A, Bending kinetics of wild-type (WT) and tir1 afb2 afb3 hypocotyls. B, Bending angle of dimethyl sulfoxide- (DMSO) or 10 μm PEO-IAA-treated wild-type hypocotyls after 24 h gravistimulation. C, Bending angle of heat shock-induced HS::axr3-1 hypocotyls after 24 h gravistimulation. D to F, PIN3-GFP localization after 24 h gravistimulation in wild-type hypocotyls upon DMSO treatment (D) and 10 μm PEO-IAA treatment (E) and in heat shock-induced HS::axr3-1 hypocotyls (F). Arrowheads depict PIN3-GFP at the outer side of endodermal cells, and the arrow in F indicates the gravity direction and hence the lower and upper sides of the hypocotyl. Scale bars = 20 μm. G and H, Quantification of PIN3-GFP intensity in PEO-IAA-treated wild-type hypocotyls (G) and heat shock-induced HS::axr3-1 hypocotyls (H) after 24 h gravistimulation. The ratio was calculated by dividing the PIN3-GFP intensity at the outer side of endodermal cells between the lower and upper sides of hypocotyls. Data and error bars represent the means ± sd. n = 30 to 40 for the bending assay and 15 for PIN3-GFP intensity quantification; **P < 0.05, determined by Student’s t test.