Figure 5.

Copresence of OsNAR2.1, OsNIT1m, and OsNIT2 improves OsNIT1-mediated IAN hydrolysis. A, Interaction test between OsNIT1 and OsNIT2 using the DUAL Membrane Pairwise Interaction Kit (Dualsystems Biotech). B, OsNIT1 and OsNIT2 enzyme activity. IAN conversion rate (consumed IAN content/total IAN content × 100%) was used as a measure of enzymatic activity by HPLC. Reaction time was 1 h at 28°C. Both the 6×His and MBP tag proteins were used as negative controls. The data represent means ± se of four biological replicates (different lowercase letters represent statistically significant difference at P ≤ 0.05 level). ND, not detectable.