Figure 4.
PA levels are not affected by PLDγ1 depletion. Five-day-old seedlings of wild-type (WT), pldγ1-1, pldγ1-2, or bir2 lines were labeled with 32Pi for 16 h and then treated for 15 min with either 1 μm flg22 (A) or 300 mm NaCl (B) or cell-free medium (control) as indicated. Lipids were extracted and separated by EtAc-TLC, and the radioactivity incorporated into PA was quantified by phosphoimaging (bottom). Data (top) represent the average ± sd of two to three biological replicates and are expressed in relation to the radioactivity of the total phospholipids. The experiment was repeated twice with similar results.