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PLDγ1 colocalizes with BIR2 and BIR3 at the PM. A, PLDγ1 was transiently expressed in N. benthamiana as GFP-fusion either alone or together with BIR2-RFP or BIR3-CFP in the presence of the p19 suppressor of silencing. Infiltration of p19 alone served as control. Fluorescence in epidermal cells was monitored at day 3 using confocal laser-scanning microscopy. B, Close-up view of the samples shown in A.
