Figure 6.

PLDγ1-GFP associates with native BIR2, but not FLS2 or BAK1. Leaf material was harvested from pldγ1-1 plants, the complementation lines 12-4 and 11-13 (pldγ1-1/35S:: PLDγ1-GFP), or wild-type (WT) plants 5 min after treatment with 1 μm flg22 (+) or water (−). After protein extraction the proteins (input) were subjected to immunoprecipitation with GFP-beads (GFP-IP). Immunoprecipitated and copurified proteins were detected with GFP antibodies or specific antibodies against native proteins as indicated. Ponceau S Red-staining of the membrane served as a loading control. M indicates the marker band at 55 kD. The experiment was repeated with similar results. RBC, Ribulose-bis-phosphate-carboxylase large subunit.