Table 3. Leaf HPR1 activity of Col-0, hpr1-1, and complemented lines (Compl-HPR1, Compl-T335A, and Compl-T335D) with different substrate-cofactor combinations.
Plants were grown in ambient air for 4 weeks before rosette leaves were collected and analyzed. Mean activities ± sd (µmol min−1 mg−1 protein) from three independent experiments, each using four different plants per line (n = 12). Values in bold are significantly different from Col-0 values (Student’s t test, P < 0.05).
| Genotype | Hydroxypyruvate:NADH | Hydroxypyruvate:NADPH | Glyoxylate:NADH | Glyoxylate:NADPH |
|---|---|---|---|---|
| Col-0 | 1.02 ± 0.08 | 0.10 ± 0.03 | 0.13 ± 0.02 | 0.04 ± 0.02 |
| Compl-HPR1-13 | 1.93 ± 0.57 | 0.15 ± 0.06 | 0.21 ± 0.06 | 0.06 ± 0.03 |
| Compl-HPR1-14 | 0.66 ± 0.27 | 0.06 ± 0.01 | 0.05 ± 0.02 | 0.03 ± 0.01 |
| Compl-HPR1-15 | 0.90 ± 0.06 | 0.08 ± 0.02 | 0.09 ± 0.02 | 0.04 ± 0.02 |
| Compl-T335A-23 | 0.90 ± 0.08 | 0.07 ± 0.02 | 0.08 ± 0.03 | 0.03 ± 0.01 |
| Compl-T335A-9 | 1.21 ± 0.04 | 0.12 ± 0.04 | 0.12 ± 0.03 | 0.04 ± 0.02 |
| Compl-T335A-21 | 0.80 ± 0.15 | 0.06 ± 0.01 | 0.06 ± 0.04 | 0.03 ± 0.01 |
| Compl-T335D-4 | 0.60 ± 0.11 | 0.15 ± 0.02 | 0.18 ± 0.04 | 0.08 ± 0.06 |
| Compl-T335D-13 | 0.60 ± 0.34 | 0.25 ± 0.13 | 0.43 ± 0.23 | 0.08 ± 0.07 |
| Compl-T335D-16 | 0.57 ± 0.08 | 0.15 ± 0.01 | 0.17 ± 0.01 | 0.08 ± 0.05 |
| hpr1-1 | 0.14 ± 0.04 | 0.03 ± 0.00 | 0.03 ± 0.02 | 0.03 ± 0.01 |