Table 6. Conditions used to measure HPR activities.
Protein Sample | Enzyme Activity | |||
---|---|---|---|---|
NADH-HP | NADPH-HP | NADH-glyoxylate | NADPH-glyoxylate | |
Recombinant HPR1 | 30–2,000 μm HP | 0.5–20 mm HP | 0.625–40 mm glycolate | 5–150 mm glycolate |
200 μm NADH | 750 μm NADPH | 200 μm NADH | 1 mm NADH | |
Recombinant HPR1 | 6.25–400 μm NADH | 12.5–500 μm NADPH | 25–800 μm NADH | 25–500 μm NADPH |
500 μm HP | 15 mm HP | 50 mm glyoxylate | 30 mm glyoxylate | |
Leaf extract | 400 μm NADH | 400 μm NADPH | 400 μm NADH | 400 μm NADPH |
1.5 mm HP | 7.5 mm HP | 50 mm glyoxylate | 50 mm glyoxylate | |
30 μg protein | 30 μg protein | 60 μg protein | 100 μg protein |