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. 2020 Mar 10;183(1):194–205. doi: 10.1104/pp.19.01225

Table 6. Conditions used to measure HPR activities.

Protein Sample Enzyme Activity
NADH-HP NADPH-HP NADH-glyoxylate NADPH-glyoxylate
Recombinant HPR1 30–2,000 μm HP 0.5–20 mm HP 0.625–40 mm glycolate 5–150 mm glycolate
200 μm NADH 750 μm NADPH 200 μm NADH 1 mm NADH
Recombinant HPR1 6.25–400 μm NADH 12.5–500 μm NADPH 25–800 μm NADH 25–500 μm NADPH
500 μm HP 15 mm HP 50 mm glyoxylate 30 mm glyoxylate
Leaf extract 400 μm NADH 400 μm NADPH 400 μm NADH 400 μm NADPH
1.5 mm HP 7.5 mm HP 50 mm glyoxylate 50 mm glyoxylate
30 μg protein 30 μg protein 60 μg protein 100 μg protein